全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
PLOS ONE  2014 

An Alternative Chemical Redox Method for the Production of Bispecific Antibodies: Implication in Rapid Detection of Food Borne Pathogens

DOI: 10.1371/journal.pone.0091255

Full-Text   Cite this paper   Add to My Lib

Abstract:

Bi-functional antibodies with the ability to bind two unrelated epitopes have remarkable potential in diagnostic and bio-sensing applications. In the present study, bispecific antibodies that recognize human red blood cell (RBC) and the food borne pathogen Listeria monocytogenes (L. monocytogenes) were engineered. The procedure involves initial reduction of a mixture of anti-RBC and anti-Listeria antibodies followed by gradual re-oxidation of the reduced disulphides. This facilitates association of the separated antibody chains and formation of hybrid immunoglobulins with affinity for the L. monocytogenes and human RBC. The bispecific antibodies caused the agglutination of the RBCs only in the presence of L. monocytogenes cells. The agglutination process necessitated the specific presence of L. monocytogenes and the red colored clumps formed were readily visible with naked eyes. The RBC agglutination assay described here provides a remarkably simple approach for the rapid and highly specific screening of various pathogens in their biological niches.

References

[1]  Holliger P, Hudson PJ (2005) Engineered antibody fragments and the rise of single domains. Nat Biotechnol 23: 1126–1136. doi: 10.1038/nbt1142
[2]  Zhang J, Tanha J, Hirama T, Khieu NH, To R, et al. (2004) Pentamerization of single-domain antibodies from phage libraries: a novel strategy for the rapid generation of high-avidity antibody reagents. J Mol Biol 335: 49–56. doi: 10.1016/j.jmb.2003.09.034
[3]  Hussack G, Luo Y, Veldhuis L, Hall JC, Tanha J, et al. (2009) Multivalent anchoring and oriented display of single-domain antibodies on cellulose. Sensors 9: 5351–5367. doi: 10.3390/s90705351
[4]  De Jonge J, Heirman C, de Veerman M, van Meirvenne S, Moser M, et al. (1998) In vivo retargeting of T cell effector function by recombinant bispecific single chain Fv (anti-CD3 x anti-idiotype) induces long-term survival in the murine BCL1 lymphoma model. J Immunol 161: 1454–1461.
[5]  Carlring J, Leenheer ED, Heath AW (2011) A novel redox method for rapid production of functional bi-specific antibodies for use in early pilot studies. PLoS ONE 6: e22533. doi: 10.1371/journal.pone.0022533
[6]  Menard S, Canevari S, Colnaghi MI (1989) Hybrid antibodies in cancer diagnosis and therapy. Int J Biol Markers 4: 131–134.
[7]  Suresh MR, Cuello AC, Milstein C (1986) Bispecific monoclonal antibodies from hybrid hybridomas. Methods Enzymol 121: 210–228. doi: 10.1016/0076-6879(86)21019-8
[8]  Lu D, Zhang H, Koo H, Tonra J, Balderes P, et al. (2005) A fully human recombinant IgG-like bispecific antibody to both the epidermal growth factor receptor and the insulin-like growth factor receptor for enhanced antitumor activity. J Biol Chem 280: 19665–19672. doi: 10.1074/jbc.m500815200
[9]  Orcutt KD, Ackerman ME, Cieslewicz M, Quiroz E, Slusarczyk AL, et al. (2010) A modular IgG-scFv bispecific antibody topology. Protein Eng Des Sel 23: 221–228. doi: 10.1093/protein/gzp077
[10]  Shen J, Vil MD, Jimenez X, Iacolina M, Zhang H, et al. (2006) Single variable domain-IgG fusion. A novel recombinant approach to Fc domain-containing bispecific antibodies. J Biol Chem 281: 10706–10714. doi: 10.1074/jbc.m513415200
[11]  Wu C, Ying H, Grinnell C, Bryant S, Miller R, et al. (2007) Simultaneous targeting of multiple disease mediators by a dual-variable-domain immunoglobulin. Nat Biotechnol 25: 1290–1297. doi: 10.1038/nbt1345
[12]  Hamon M, Bierne H, Cossart P (2006) Listeria monocytogenes: a multifaceted model. Nat Rev Microbiol 4: 423–434. doi: 10.1038/nrmicro1413
[13]  Guttikonda S, Wang W, Suresh M (2004) Molecular zipper assays: a simple homosandwich with the sensitivity of PCR. J Pharm Pharmaceut Sci 7: 7–16.
[14]  Allos BM, Moore MR, Griffin PM, Tauxe RV (2004) Surveillance for sporadic foodborne disease in the 21st century: the FoodNet perspective. Clin Infect Dis 38: S115–S120. doi: 10.1086/381577
[15]  Padmapriya P, Banada, Bhunia AK (2008) Antibodies and Immunoassays for Detection of Bacterial Pathogens. Principles of bacterial detection: biosensors, recognition receptors and Microsystems III: 567–602. doi: 10.1007/978-0-387-75113-9_21
[16]  Hamon M, Bierne H, Cossart P (2006) Listeria monocytogenes: a multifaceted model. Nat Rev Microbiol 4(6): 423–434. doi: 10.1038/nrmicro1413
[17]  Gasanov U, Hughes D, Hansbro PM (2005) Methods for the isolation and identification of Listeria spp. and Listeria monocytogenes: A review. FEMS Microbiol Rev 29: 851–875. doi: 10.1016/j.femsre.2004.12.002
[18]  Dodge JT, Mitchell C, Hanahan DJ (1963) The preparation and chemical characteristics of haemoglobin free ghost of human erythrocytes. Arch Biochem Biophys 100: 119–130. doi: 10.1016/0003-9861(63)90042-0
[19]  Iglesias BF, Catala A (2005) Rat, equine and bovine erythrocyte ghosts exposed to t-butyl hydroperoxide as a model to study lipid peroxidation using a chemiluminescence assay. Res Vet Sci 79: 19–27. doi: 10.1016/j.rvsc.2004.10.004
[20]  Mujahid S, Pechan T, Wang C (2007) Improved solubilisation of surface proteins from Listeria monocytogenes for 2-DE. Electrophoresis 28: 3998–4007. doi: 10.1002/elps.200600858
[21]  Müller S, Hain T, Pashalidis P, Lingnau A, Domann E, et al. (1998) Purification of the inlB gene product of Listeria monocytogenes and demonstration of its biological activity. Infect Immun. 66(7): 3128–33.
[22]  Likhite N, Warawdekar UM (2011) A Unique Method for Isolation and Solubilization of Proteins after Extraction of RNA from Tumor Tissue Using Trizol. J Biomol Tech 22: 37–44.
[23]  Bratcher RL, Chong CA, Dray S (1974) The isolation & characterization of an antisheep red blood cell antibody having limited heterogeneity. J Immunol 112: 1337–1346.
[24]  Singhal A, Bali A, Gupta CM (1986) Antibody-mediated targeting of liposomes to erythrocytes in whole blood. Biochem Biophys Acta 880: 72–77. doi: 10.1016/0304-4165(86)90121-2
[25]  Porath J, Olin B, Granstrand B (1983) Immobilized metal affinity chromatography of serum proteins on gel immobilized group III A metal ions. Arch Biochem Biophys 225: 543–547. doi: 10.1016/0003-9861(83)90065-6
[26]  Chauhan A, Zubair S, Tufail S, Sherwani A, Sajid M, et al. (2011) Fungus-mediated biological synthesis of gold nanoparticles: potential in detection of liver cancer. Int J Nanomedicine 6: 2305–2319. doi: 10.2147/ijn.s23195
[27]  Ohk SH, Koo OK, Sen T, Yamamoto CM, Bhunia AK (2010) Antibody-aptamer functionalized fibre-optic biosensor for specific detection of Listeria monocytogenes from food. J Appl Microbiol 109: 808–17. doi: 10.1111/j.1365-2672.2010.04709.x
[28]  Kendall PA, Hillers VV, Medeiros LC (2006) Food safety guidance for older adults. Clin Infect Dis 42: 1298–1304. doi: 10.1086/503262
[29]  CDC. Update on multi-state outbreak of E. coli O157:H7 infections from fresh spinach. (2006) In: Department of Health and Human Services (ed) E. coli O157:H7 Outbreak in Spinach. Center for Disease control and Prevention (CDC), Atlanta, Georgia. Available: http://www.cdc.gov/foodborne/ecolispinac?h/100606.htm.
[30]  Bhunia AK (2006). Detection of significant bacterial pathogens and toxins of interest in homeland security. In: Amass SF, Bhunia AK, Chaturvedi AR, Dolk DR, Peeta S, and Atallah MJ, (eds) The Science of Homeland Security. Purdue University Press, West Lafayette, Indiana. pp. 109–149.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133