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PLOS ONE  2014 

Comparative Protein Composition Analysis of Goat Milk Produced by the Alpine and Saanen Breeds in Northeastern Brazil and Related Antibacterial Activities

DOI: 10.1371/journal.pone.0093361

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Abstract:

The protein composition of goat milk differs between goat breeds and could present regional trends. The aim of this study was to comparatively analyze the protein composition of goat milk produced by the Alpine and Saanen breeds in northeastern Brazil and to evaluate the antibacterial activity of its protein fractions. SDS-PAGE, 2-DE electrophoresis and RP-HPLC analyses revealed the absence of αs1-casein in the milk of both breeds and no differences between the αs2-casein, β-casein, β-lactoglobulin and α-lactalbumin profiles. The amounts of soluble proteins and β-casein hydrolysis residues were higher in Saanen milk. Only the protein fraction containing the largest amounts of casein (F60–90%) inhibited bacterial growth, with MIC values between 50 and 100 mg/mL. This study describe for the first time three important points about the goat milk protein of two Brazilian goat breeders: absence of α-s1 casein in the protein profile, differences between the milk protein composition produced by goats of Alpine and Saanen breeders and antibacterial activity of unbroken proteins (casein-rich fraction) present in these milk.

References

[1]  Drewnowski A, Fulgoni V (2008) Nutrient profiling of foods: creating a nutrient-rich food index. Nutr Rev 66: 23–39. doi: 10.1111/j.1753-4887.2007.00003.x
[2]  Santos A, Dias A, Pinheiro Já (2010) Predictive factors for the persistence of cow's milk allergy. Pediatr Allergy Immu 21: 1127–1134. doi: 10.1111/j.1399-3038.2010.01040.x
[3]  Haenlein GFW (2004) Goat milk in human nutrition. Small Rum Res 51: 155–163. doi: 10.1016/j.smallrumres.2003.08.010
[4]  Vargas M, Cháfer M, Albors A, Chiralt A, González-Martinéz C (2008) Physicochemical and sensory characteristics of yogurt produced from mixtures of cow’s and goat’s milk. Int Dairy J 18: 1142–1152. doi: 10.1016/j.idairyj.2008.06.007
[5]  Olalla M, Ruiz-López MD, Navarro M, Artacho R, Cabrera C, et al. (2009) Nitrogen fractions of Andalusian goat milk compared to similar types of commercial milk. Food Chem 113: 835–838. doi: 10.1016/j.foodchem.2008.10.022
[6]  Marletta D, Bordonaro S, Guastella AM, D’Urso G (2009) Genetic polimorphism at CSN1S2 locus in two endangered Sicilian goat breeds. J Anim Breed Genet 121: 52–56. doi: 10.1046/j.0931-2668.2003.00413.x
[7]  Prinzenberg EM, Gutscher K, Chessa S, Caroli A, Erhardt G (2005) Caprine kappa-casein (CSN3) polymorphism: New developments in molecular knowledge. J Dairy Sci 88: 1490–1498.
[8]  Ceballos LS, Morales ER, Adarve GT, Castro JD, Martinez LP, et al. (2009) Composition of goat and cow milk produced under similar conditions and analyzed by identical methodology. J Food Comp Anal 22: 322–329. doi: 10.1016/j.jfca.2008.10.020
[9]  Park YW, Juarez M, Ramos M, Haenlein GFW (2007) Physico-chemical characteristics of goat and sheep milk. Small Rum Res 68: 88–113. doi: 10.1016/j.smallrumres.2006.09.013
[10]  Eriksen EK, Vegarud GE, Langsrud T, Almaas H, Lea T (2008) Effect of milk proteins their hydrolysates on in vitro immune responses. Small Rum Res 79: 29–37. doi: 10.1016/j.smallrumres.2008.07.003
[11]  Roncada P, Piras C, Soggiu A, Turk R, Urbani A, et al. (2012) Farm animal milk proteomics. J Proteomics 75: 4259–4274. doi: 10.1016/j.jprot.2012.05.028
[12]  Murata M, Wakabayashi H, Yamauchi K, Abe F (2013) Identification of milk proteins enhancing the antimicrobial activity of lactoferrin and lactoferricin. J Dairy Sci 96: 4891–4898.
[13]  Moatsou G, Moschopoulou E, Mollé D, Gagnaire V, Kandarakis I, et al. (2008) Comparative study of the protein fraction of goat milk from the Indigenous Greek breed and from international breeds. Food Chem 106: 509–520. doi: 10.1016/j.foodchem.2007.06.014
[14]  Maga EA, Daftari P, Kültz D, Penedo MCT (2009) Prevalence of αs1-casein genotypes in American dairy goats. J Anim Sci 87: 3464–3469. doi: 10.2527/jas.2009-1854
[15]  Hinz K, O’Connor PM, Huppertz T, Ross RP, Kelly AL (2012) Comparison of the principal proteins in bovine, caprine, buffalo, equine and camel milk. J Dairy Res 79: 185–191. doi: 10.1017/s0022029912000015
[16]  López-Exposito I, Pellegrini A, Amigo L, Recio I (2008) Synergistic effect between different milk-derived peptides and proteins. J Dairy Sci 91: 2184–2189.
[17]  Meisel H, Schlimme E (1996) Bioactive peptides derived from milk proteins: ingredients for functional foods. Kieler Milchw Forsch 48: 343–357.
[18]  Pihlanto A, Korhonen H (2003) Bioactive peptides and proteins. Adv Food Nutr Res 47: 175–276. doi: 10.1016/s1043-4526(03)47004-6
[19]  Almaas H, Berner V, Holm H, Langsrud T, Vegarud GE (2008) Degradation of whey from caprine milk by human proteolytic enzymes, and the resulting antibacterial effect against Listeria monocytogenes. Small Rum Res 79: 11–15. doi: 10.1016/j.smallrumres.2008.07.013
[20]  AOAC International – Association of Analytical Communities (2012) Official Methods of Analysis. (19th ed.). Gaithersburg, MD.
[21]  APHA – American Public Health Association (2001) Compendium of methods for the microbiological examination of foods. (4th ed.). Washington, (Chapter 7).
[22]  Brazil. National Health Surveillance Agency/Ministry of Health (ANVISA/MS). RDC Resolution 12, january 2nd, 2001. Approves the Technical Regulation on Microbiological Standards for Foods. Official Federal Gazette; Government, january 10th 2001. URL: http://www.anvisa.gov.br/e-legis/. Accessed 12 June 13.
[23]  Scopes RK (1994) Protein purification: principles and practices. Spring advanced texts in chemistry. (3rd ed.). New York: Springer Verlag.
[24]  Egito AS, Rosinha GMS, Laguna LE, Miclo L, Girardet JM, et al. (2006) Fast electrophoretic detection method of adulteration of caprine milk by bovine milk. Braz J Vet Anim Sci 58: 932–939.
[25]  Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the Principle of Protein-Dye Binding. Anal Biochem 72: 248–254. doi: 10.1006/abio.1976.9999
[26]  Jaubert A, Martin P (1992) Reverse-phase HPLC analysis of goat caseins. Identification of αs1 and αs2 genetic variants. Lait 72: 235–247. doi: 10.1051/lait:1992317
[27]  Neveu C, Mollé D, Moreno J, Martin P, Léonil J (2002) Heterogeneity of caprine beta-casein elucidated by RP-HPLC/MS: Genetic variants and phosphorylations. J Protein Chem 21: 557–567.
[28]  Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685. doi: 10.1038/227680a0
[29]  O'Farrel PH, Klose J (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 25: 4007–4021.
[30]  Wang FX (2011) Proteomic analysis of the sea-island cotton roots infected by wilt pathogen Verticillium dahliae. Proteomics 11(22): 4296–4309. doi: 10.1002/pmic.201100062
[31]  Demers-Mathieu V, Gauthier SF, Britten M, Fliss I, Robitaille G, et al. (2013) Antibacterial activity of peptides extracted from tryptic hydrolyzated of whey protein by nanofiltration. Int Dairy J 28: 94–101. doi: 10.1016/j.idairyj.2012.09.003
[32]  NCCLS – National Committee for Clinical Laboratory Standards (2003) Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard (6th ed.). NCCLS document M7-A6 (ISBN 1-56238-486-4). NCCLS, 940 West Valley Road, Suite 1400, Wayne, Pennsylvania 19087-1898 USA.
[33]  Moatsou G, Samolada M, Panagiotou P, Anifantakis E (2004) Casein fraction of bulk milks from different caprine breeds. Food Chem 87: 75–81. doi: 10.1016/j.foodchem.2003.10.020
[34]  Queiroga RCRE, Costa RG, Biscotini TMB, Medeiros AN, Madruga MS, et al. (2007) Effects of flock management, milking sanitary conditions and lactation stage on milk composition of Saanen goats. Braz J Anim Sci 36: 430–437.
[35]  Bevilacqua C, Martin P, Candalh C, Fauquant J, Piot M, et al. (2001) Goat’s milk defective αs1-casein decreases intestinal and systemic sensitization to β-lactoglobulin in guinea pig. J Dairy Res 67: 217–227. doi: 10.1017/s0022029901004861
[36]  Lara-Villoslada F, Olivares M, Xaus J (2005) The balance between caseins and whey proteins in cow’s milk determines its allergenicity. J Dairy Sci 88: 1654–1660.
[37]  Silva MMC, Torres RA, Rodrigues MT, Soares MAM, Magalh?es ACM, et al. (2009) Effect of genotypes for αs1-casein on proteic and lipidic fractions in goat milk. Braz J Vet Anim Sci 61: 682–690.
[38]  Trujillo AJ, Guamis B, Carretero C (1997) Hydrolysis of caprine β-casein by plasmin. J Dairy Res 80: 2258–2263.
[39]  Santillo A, Kelly AL, Palermo C, Sevi A, Albenzio M (2009) Role of indigenous enzymes in proteolysis of casein in caprine milk. Int Dairy J 19: 655–660. doi: 10.1016/j.idairyj.2009.06.011
[40]  Albenzio M, Caroprese M, Marino R, Muscio A, Santillo A, et al. (2006) Characteristics of Garnica goat milk and Cacioricotta cheese. Small Rumin Res 64: 35–44. doi: 10.1016/j.smallrumres.2005.03.010
[41]  Mckenzie HA, White FHJ (1991) Lysozyme and α-lactalbumin: Structure, function and interrelationships. Adv Protein Chem 41: 173–315. doi: 10.1016/s0065-3233(08)60198-9
[42]  Sgarbieri VC (2005) Review: Structural properties and physico-chemical properties of milk proteins. Braz J Food Tech 8: 43–56.
[43]  Sardina MT, Rosa AJM, Davoli R, Braglia S, Portolano B (1992) Polymorphisms of beta-lactoglobulin promoter region in three Sicilian goat breeds. Mol Biol Rep 39: 3203–3210. doi: 10.1007/s11033-011-1087-5
[44]  Erdem YK (2000) Influence of ulltrafiltration on modification of surface hydrophobic sites of the milk protein system in the course of renneting. J Food Eng 44: 63–70. doi: 10.1016/s0260-8774(99)00165-x
[45]  Yuksel Z, Avci E, Uymaz B, Erdem YK (2012) General composition and protein surface hydrophobicity of goat, sheep and cow milk in the region of Mount Ida. Small Rumin Res 106: 137–144. doi: 10.1016/j.smallrumres.2012.03.022
[46]  Silanikove N, Leitner G, Merin U, Prosser CG (2010) Recent advances in exploiting goat’s milk: Quality, safety and production aspects. Small Rumin Res 89: 110–124. doi: 10.1016/j.smallrumres.2009.12.033
[47]  López-Expósito I, Recio I (2006) Antibacterial activity of peptides and folding variants from milk proteins. Int Dairy J 16: 1294–1305. doi: 10.1016/j.idairyj.2006.06.002

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