We develop a new hidden Markov model-based method to analyze C elegans locomotive behavior and use this method to quantitatively characterize behavioral states. In agreement with previous work, we find states corresponding to roaming, dwelling, and quiescence. However, we also find evidence for a continuum of intermediate states. We suggest that roaming, dwelling, and quiescence may best be thought of as extremes which, mixed in any proportion, define the locomotive repertoire of C elegans foraging and feeding behavior.
References
[1]
Shtonda BB, Avery L (2006) Dietary choice behavior in Caenorhabditis elegans. J Exp Biol 209: 89–102.
[2]
Williams KW, Elmquist JK (2012) From neuroanatomy to behavior: central integration of peripheral signals regulating feeding behavior. Nat Neurosci 15: 1350–1355.
[3]
Avery L, You YJ (2012) C. elegans feeding. WormBook: 1–23.
[4]
Ashrafi K (2007) Obesity and the regulation of fat metabolism. WormBook: 1–20.
[5]
You YJ, Avery L (2012) Appetite control: worm’s-eye view. Animal Cells and Systems: 1–6.
[6]
Fujiwara M, Sengupta P, McIntire SL (2002) Regulation of body size and behavioral state of C. elegans by sensory perception and the EGL-4 cGMP-dependent protein kinase. Neuron 36: 1091–1102.
[7]
Ben Arous J, Laffont S, Chatenay D (2009) Molecular and sensory basis of a food related two-state behavior in C. elegans. PLoS One 4: e7584.
[8]
Hills T, Brockie PJ, Maricq AV (2004) Dopamine and glutamate control area-restricted search behavior in Caenorhabditis elegans. J Neurosci 24: 1217–1225.
[9]
Van Buskirk C, Sternberg PW (2007) Epidermal growth factor signaling induces behavioral quiescence in Caenorhabditis elegans. Nat Neurosci 10: 1300–1307.
[10]
Raizen DM, Zimmerman JE, Maycock MH, Ta UD, You YJ, et al. (2008) Lethargus is a Caenorhabditis elegans sleep-like state. Nature 451: 569–572.
[11]
You YJ, Kim J, Raizen DM, Avery L (2008) Insulin, cGMP, and TGF-beta signals regulate food intake and quiescence in C. elegans: a model for satiety. Cell Metab 7: 249–257.
[12]
Avery L (1993) The genetics of feeding in Caenorhabditis elegans. Genetics 133: 897–917.
[13]
Shoval O, Sheftel H, Shinar G, Hart Y, Ramote O, et al.. (2012) Evolutionary Trade-Offs, Pareto Optimality, and the Geometry of Phenotype Space. Science.
[14]
Sawin ER, Ranganathan R, Horvitz HR (2000) C. elegans locomotory rate is modulated by the environment through a dopaminergic pathway and by experience through a serotonergic pathway. Neuron 26: 619–631.
[15]
Chen S, Lee AY, Bowens NM, Huber R, Kravitz EA (2002) Fighting fruit flies: a model system for the study of aggression. Proc Natl Acad Sci U S A 99: 5664–5668.
[16]
Gottman JM, Roy AK (1990) Sequential analysis : a guide for behavioral researchers. Cambridge England; New York: Cambridge University Press. ix, 275 p. p.
[17]
Srivastava N, Clark DA, Samuel AD (2009) Temporal analysis of stochastic turning behavior of swimming C. elegans. J Neurophysiol 102: 1172–1179.
[18]
Carola V, Mirabeau O, Gross CT (2011) Hidden Markov model analysis of maternal behavior patterns in inbred and reciprocal hybrid mice. PLoS One 6: e14753.
[19]
Yamato J, Ohya J, Ishii K (1992) Recognizing human action in time-sequential images using hidden Markov model. Proceedings CVPR ’92, 1992 IEEE Computer Society Conference on Computer Vision and Pattern Recognition, 1992 379–385.
[20]
Stephens GJ, Johnson-Kerner B, Bialek W, Ryu WS (2008) Dimensionality and dynamics in the behavior of C. elegans. PLoS Comput Biol 4: e1000028.
[21]
Piggott BJ, Liu J, Feng Z, Wescott SA, Xu XZ (2011) The neural circuits and synaptic mechanisms underlying motor initiation in C. elegans. Cell 147: 922–933.
[22]
Ben Arous J, Tanizawa Y, Rabinowitch I, Chatenay D, Schafer WR (2010) Automated imaging of neuronal activity in freely behaving Caenorhabditis elegans. J Neurosci Methods 187: 229–234.
[23]
Zheng M, Cao P, Yang J, Xu XZ, Feng Z (2012) Calcium imaging of multiple neurons in freely behaving C. elegans. J Neurosci Methods 206: 78–82.
[24]
Johnson NL (1949) Systems of frequency curves generated by methods of translation. Biometrika 36: 149–176.
[25]
Blattberg R, Gonedes N (1974) A comparison of the stable and student distributions as statistical models for stock prices. J Business 47: 244–280.
[26]
Press WH (2007) Numerical recipes : the art of scientific computing. Cambridge, UK; New York: Cambridge University Press. xxi, 1235 p.
[27]
Caprio MA (2005) LevelScheme: A level scheme drawing and scientific figure preparation system for Mathematica. Computer Physics Communications 171: 107–118.
