HEAT SHOCK PROTEIN AS OXIDATIVE SRESS MARKERS IN RATS SUBIMITTED TO EXHAUSTIVE INTERMITTENT RUNNING TRAINING

ANTUNES NETO, J. M. F.; PEREIRA-DA-SILVA, l.; MACEDO, D. V. Heat Shock Proteins as Oxidative Stress Markers in Rats Submitted to Exhaustive Intermittent Running Training. Brazilian Journal of Biomotricity, v. 2, n. 3, p. 160-175, 2008. A novel method to measure oxidative stress resulting from exhaustive Intermittent training in rats submitted to the treadmill running is presented. In this new procedure we evaluated the erythrocyte antioxidant enzymes, catalase (CAT) and glutathione reductase (GR), the plasma oxidative attack markers, reactive carbonyl derivatives (RCD) and thiobarbituric reactive substances (TBARS). Muscular tissue damage was evaluated by monitoring plasma creatine kinase (CK) and aspartate transaminase (AST) concentrations. Also, we monitored total the level of the 70 Kda heat shock protein (HSP70) in leukocytes as a marker of oxidative stress. In the study we found a correspondence between oxidative stress markers and leukocyte HSP70 levels. The results showed that the adaptation phase induced an increase of antioxidant activity and stabilization in levels of oxidative attack markers; the HSP70 levels had modulation in accordance with the training conditions. However, when the rats had 3 and 4 sessions of training per day and a decrease in time of recovery (overreaching phase), the antioxidant activity suffered a decrease and the oxidative attack markers had a significative increase. As a response to the severity of the oxidative stress condition, the HSP70 levels had a significative increase, supporting the hypothesis that the HSPs acts as an important element of the cells antioxidant defenses.