Statistical analysis of cellulase production in Bacillus amyloliquefaciens UNPDV-22

The production of cellulase in Bacillus amyloliquefaciens UNPDV-22 was optimized usingresponse surface methodology (RSM). Central composite design (CCD) was used to study the interactiveeffect of fermentation medium components (wheat bran, soybean meal, and malt dextrin) on cellulaseactivity. Results suggested that wheat bran, soybean meal, and malt dextrin all have significant impacton cellulase production. The use of RSM resulted in a 70% increase in the cellulase activity over thecontrol of non-optimized basal medium. Optimum cellulase production of 11.23 U/mL was obtained in afermentation medium containing wheat bran (1.03%, w/v), soybean meal (2.43%, w/v), and maltdextrin (2.95%, w/v).