HPLC methods for the determinatiuon of acetyl- and benzoyl-tiazofurin in rat plasma

Reverse-phase HPLC methods for determination of 5 -O-acetyl-tiazofurin (AT) and 5 -O-benzoyl-tiazofurin (BT) in rat plasma were developed and validated in terms of specificity, linearity, precision, accuracy and sensitivity. Linear calibration curves were constructed in the concentration range of 2.50 100.00 mmol/L for both compounds. The separations were achieved on a Supelcosyl LC-18-DB analytical column (250×4.6 mm; 5 mm particle size) by isocratic elution, with a mixture of 0.1 M disodium hydrogen phosphate methanol. UV detection was performed at a wavelength of 254 nm. The proposed methods enable the detection and quantification of nanomolar concentrations of AT and BT in rat plasma.