大鼠背侧脑桥及腹侧脑桥神经元NMDA受体在黑伯反射中的作用
Role of NMDA Receptors Located in Dorsolateral-Pons and Ventrolateral-Pons Neurons in Hering-Breuer Reflex

DOI: 10.12677/HJBM.2012.24008, PP. 31-35

Keywords: 黑伯反射；背侧脑桥；腹侧脑桥；大鼠
Hering-Breuer Reflex, Dorsolateral-Pons, Ventrolateral-Pons, Rat

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Abstract:

黑伯反射又称为肺牵张感受器反射，是肺牵张感受器兴奋所导致的呼气延长与吸气缩短反应。电刺激迷走神经中枢端可以模拟该反射。我们前期研究发现，在腹侧脑桥，NMDA受体介导的递质传递可以对黑伯反射产生调节作用。本研究在前期研究基础上，以大鼠为实验对象，通过电刺激其迷走神经中枢端诱导出黑伯反射，分别向背侧脑桥及腹侧脑桥内微量注射NMDA受体阻断剂D-2-氨基-5-磷酸基戊酸(D-AP5)，观察药物对黑伯反射的调节作用。结果表明，向背侧脑桥注射D-AP5后，黑伯反射的呼吸抑制效应明显增强，向腹侧脑桥注射D-AP5，黑伯反射的呼吸抑制效应明显削弱。NMDA受体介导的递质传递在背侧脑桥及腹侧脑桥对黑伯反射发挥方向相反的调节作用。
Vagal stimulation evokes Hering-Breuer Reflex (HBR), which is characterized by inspiratory inhibition and expiratory prolongation. In this work, the role of NMDA receptors located in dorsolateral-pons and ventrolateral-pons were studied and compared by comparing the strength of this reflex before and after microinjecting D-AP5. Experi- ments were performed on urethane anesthetized adult rats. The HBR was simulated by electrical stimulation of the central end of cervical vagus nerve. We found that the HBR was strengthened after microinjection D-AP5 into dorsolateral-pons, while weakened after microinjection in ventrolateral-pons. This work shows that NMDA receptor mediated neurotransmissions both in dorsolateral-pons and ventrolateral-pons exert opposite effects on HBR.

References

[1]	E. S. Schelegle, J. F. Green. An overview of the anatomy and physiology of slowly adapting pulmonary stretch receptors. Respiration Physiology, 2001, 125: 17-31.
[2]	Y. Li, G. Song, C. Ying, et al. Modulation of the Hering-Breuer reflex by Raphe Pallidus in rabbits. Neuroscience Letters, 2006, 397: 259-262.
[3]	H. Wang, H. Zhang, G. Song, et al. Modulation of Hering-Breuer reflex by ventrolateral pons. Advances in Experimental Medi- cine and Biology, 2008, 605: 387-392.
[4]	汪慧, 于书彦, 张衡. 大鼠腹侧脑桥神经元NMDA受体和GABA受体在黑伯反射中的作用[J]. 山东医药, 2011, 51(20): 13-14.
[5]	M. S. Siniaia, D. L. Young and C. S. Poon. Habituation and desensitization of the Hering-Breuer reflex in rat. Journal of Physiology (London), 2000, 523(2): 479-491.
[6]	G. Paxions, C. Watson. The rat brain in stereotaxic coordinates. 6th Edition, New York: Academic Press, 2007: 456.
[7]	A. J. Berger, D. B. Averill. Projection of single pulmonary stretch receptors to solitary tract region. Journal of Neurophysi- ology, 1983, 49(3): 819-830.
[8]	A. J. Berger, T. E. Dick. Connectivity of slowly adapting pul- monary stretch receptors with dorsal medullary respiratory neurons. Journal of Neurophysiology, 1987, 58(6): 1259-1274.
[9]	K. Ezure, K. Otake, J. Lipski, et al. Efferent projections of pulmonary rapidly adapting receptor relay neurons in the cat. Brain Research, 1991, 564(2): 268-278.
[10]	K. Ezure, I. Tanaka. Pump neurons of the nucleus of the solitary tract project widely to the medulla. Neuroscience Letters, 1996, 215(2): 123-126.
[11]	K. Ezure, I. Tanaka. Identification of deflation-sensitive in- spiratory neurons in the dorsal respiratory group of the rat. Brain Research, 2000, 883(1): 22-30.
[12]	K. Otake, Y. Nakamura, I. Tanaka, et al. Morphology of pul- monary rapidly adapting receptor relay neurons in the rat. The Journal of Comparative Neurology, 2001, 430(4): 458-470.
[13]	K. Ezure, I. Tanaka. Lung inflation inhibits rapidly adapting receptor relay neurons in the rat. NeuroReport, 2000, 11(8): 1709-1712.
[14]	K. Ezure, I. Tanaka. GABA, in some cases together with glycine, is used as the inhibitory transmitter by pump cells in the Hering- Breuer reflex pathway of the rat. Neuroscience, 2004, 127(2): 409-417.
[15]	T. Lumsden. Observations on the respiratory centres in the cat. Journal of Physiology (London), 1923, 57: 153-160.
[16]	M. I. Cohen. Switching of the respiratory phases and evoked phrenic responses produced by rostral pontine electrical stimul- ation. Journal of Physiology (London), 1971, 217: 133-158.
[17]	C. Von Euler. Brain stem mechanisms for generation and control of breathing pattern. In: A. P. Fishman, N. S. Cherniack, J. G. Widdicombe and S. R. Geiger, Eds., Handbook of Physiology, Bethesda: American Physiological Society, 1986, 2(3): 1-67.
[18]	W. Wang, M. L. Fung and W. M. St. John. Pontine regulation of ventilatory activity in the adult rat. Journal of Applied Physi- ology, 1993, 74: 2801-2811.
[19]	M. L. Fung, W. Wang and W. M. St. John. Involvement of pon- tile NMDA receptors in inspiratory termination in rat. Respira- tion Physiology, 1994, 96: 177-188.
[20]	S. F. Morrison, S. L. Cravo and H. M. Wilfehrt. Pontine lesions produce apneusis in the rat. Brain Research, 1994, 652: 83-86.
[21]	M. B. Harris, W. K. Milsom. Apneusis follows disruption of NMDA-type glutamate receptors in vagotomized ground squir0 rels. Respiratory Physiology & Neurobiology, 2003, 134: 191- 207.
[22]	M. S. Siniaia, D. L. Young and C. S. Poon. Habituation and desensitization of the Hering-Breuer reflex in rat. Journal of Physiology (London), 2000, 523(2): 479-491.
[23]	J. Jodkowski, S. K. Coles and T. E. Dick. A “pneumotaxic centre” in rats. Neuroscience Letters, 1994, 172(1-2): 67-72.
[24]	M. D. Milner, J. P. Lara and M. P. L. de Miguel. A5 region modulation of the cardiorespiratory responses evoked from parabrachial cell bodies in the anaesthetized rat. Brain Research, 2003, 982(1): 108-118.
[25]	J. Jodkowski, S. K. Coles and T. E. Dick. Prolongation in expiration evoked from ventrolateral pons of adult rats. Applied Physiology, 1997, 82(2): 377-381.

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