An In Vitro Study on Effect of Lactic Acid and Ascorbic Acid on Etoposide-Induced Lipid Peroxidation

Lipid peroxidation, a free radical mediated process is involved in several injurious consequences like cardiomyopathy, neurodegenerative diseases, etc. Reactive oxygen species (ROS) is involved as a mediator of free radical related damage. Antioxidants have the capability to scavenge free radicals and protect tissues from ROS. Keeping in mind this fact the present in vitro study was carried out to evaluate the lipid peroxidation induction potential of etoposide, an antimalarial and its suppression with ascorbic acid and lactic acid using malondialdehyde (MDA) as laboratory marker. Liver homogenate was treated with drug as well as antioxidant and incubated for definite time period and MDA content was estimated in different samples. Result showed that etoposide could significantly induce lipid peroxidation and ascorbic acid and lactic acid could significantly inhibit etoposide-induced lipid peroxidation. It was further found from the study that antioxidant potential of lactic acid is more than that of ascorbic acid.