Development and Validation of a UV - Spectrophotometric Method for Quantification of Atorvastatin in Tablets

A UV-spectrophotometric method has been developed for the quantitativeestimation of atorvastatin in tablet. The present study describes development and validation of UV-spectroscopic method for estimationof atorvastatin in tablet dosage form and validation of newlydeveloped method. During development of analytical method methanol:phosphate buffer (20:80),methanol:water(20:80),methanol:water (50:50),acetonitrile:water (10:90)were tried but drug was found to be solublein methanol:water (50:50). Standard stock solution was prepared in methanol: water (50:50). The detection wave length (λmax) was found tobe 248nm.Calibration curves were prepared.The proposed method obeysBeer's law in the range of 5-15μg/ml. Absorption maxima was determinedwith 10 μg/ml by scanning in the range of 200-400nm.Percent recoverystudies are in the range of 98.78%-100.36%. The method was validated in terms specificity,linearity,accuracy,range,precision,repeatability,robustness,system suitability,stability of analytical solution.Results of analysis were validated statistically and by specificity studies.From that it was observed that there is no interference of blank,excipients during the estimation of drug in formulation. This showsthe adaptability of the method for routine estimation of atorvastatinin tablet dosage form.