Anti-Urolithiatic Activity of Dolichos Biflorus Seeds

A kidney stone, also known as a renal calculus is a solid concretion or crystal aggregation formed in the kidneys from dietary minerals in the urine. Urolithiasis is a complex process that occurs from series of several physicochemical event including super-saturation, nucleation, growth, aggregation and retention within the kidneys. Data from in-vitro, in- vivo and clinical trials reveal that phytotherapeutic agents could be useful as either alternative or an adjunct therapy in the management of Urolithiasis. Medicinal plants / natural products are more useful for body because they promote the repair mechanism in natural way. Various plant species of Dolichos biflorus, have been reported to posses antiurolithiatic property. In this study aqueous, chloroform, benzene extracts of Dolichos biflorus.Linn and standard for dissolving kidney stones- calcium oxalate by an in-vitro model. To check their potential to dissolve experimentally prepared kidney stones- calcium oxalate by an in-vitro model for Dolichos biflorus seeds and cystone as a standard compound collected from market. Phenolic compound isolated from the benzene and aqueous, flavanoids and steroids from aqueous fraction of the seed. Aqueous fractions showed highest dissolution of stones as compare to others. Aqueous fraction was more effective in dissolving calcium oxalate (48.5±0.022%). Reference standard-formulation Cystone was found to be more effective (53.5±0.02 %) when compared to phenolic and flavanoids fraction.