The Effects Of Menadione On Rat Glial Cell Proliferation

Background: Menadione inhibits cell growth of rodent and human cell lines. We reported that menadione had potential antiproliferative effect doses depend on both rat glioma (C6) and low passage human glioma cells in vitro. In this study, we examine menadione toxicity on primary glial cell culture cells in vitro.Material and Methods: Glial cells were obtained from 1-3 day old rat brain and cultured in humidified atmosphere of 5 % CO2, at 37 oC in flask. The glial cells were seeded in 96 well plates and incubated for 24 hours. Then cells were exposed 10, 25, 50, 75 ,and 100 μM menadione doses. After 24 hours, menadione toxicity was measured by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, (MTT) assay.All statistical analyses were performed by one-way analysis of variance (ANOVA) and followed by Tukey’s multiple comparison tests.Results: When the compared to the control, 10 and 25 μM menadione doses did not shown any activity on glial cell proliferation (p>0.05). However, 50, 75 ,and 100 μM menadione doses reduced 49, 61, 63 % in glial cells viability respectively (p<0.0001). Fifty % growth inhibitory concentration (IC50) was calculated as 48 μM.Conclusion: Our finding suggested that rat glial cells susceptible to menadione toxicity. In addition menadione has growth inhibitory effects in a dose depend manner on rat glial cells like rat glioma cell in vitro.