Immunohistochemical Localization of Neuron Specific Enolase and CD3 Lymphocyte Activation

Immunohistochemical localization of CD3 and Neuron Specific Enolase (NSE) to demonstrate neuronal metabolism in proliferation and cell recovery was carried out in this study to evaluate morphological and cellular changes in cyanide treated perfused cortical tissues of adult wistar rats in vitro. Four separately perfused tissues were stained with antibodies specific for neuron specific enolase using antigen retrieval method and colour reaction involving 3’3’-Di aminobenzidine tetrachloride (DAB) in peroxidase anti peroxidase method. Treated cells showed increased in enolase activity implying increase proliferation activity for repair purposes in the assaulted neuron (cyanide treated neurons). Presence of CD3 activity also indicates the presence of undifferentiated T-lymphocytes around the cells undergoing apoptosis in the cortex following treatment with cyanide.