OTIMIZA O DOS PAR METROS DE HIDRóLISE ENZIMáTICA DO BAGA O DE CANA-DE-A úCAR

Recently, the search of renewable sources of energy has grown due to the depleting of fossilfuels, increasing the possibility at the conversion of the lignocellulosic biomass via hydrolytic enzymes.The aim of this paper was to evaluate and optimize the enzymatic hydrolysis conditions of the sugarcanebagasse by cellulases produced by solid-state fermentation (SSF) with the Trichoderma sp. fungi from theamazon biome. The substrate for the cellulases production at the SSF was the sugarcane bagasse previouslypretreated. The cellulolytic extract reached an enzymatic activity of 0.162 U.mL-1 and was employed at theenzymatic hydrolysis aiming the production of fermentable sugars. Using the response surface methodologythe parameters hydrolysis time (t), enzyme dilution (D), Tween 80 concentration (C) and solid-liquid ratio(R) were evaluated at the enzymatic activity response. Another commercial enzyme was used at the essaysto establish a comparison. The higher yield of enzymatic activity was 0.324 and 0.362 U.mL-1 for theproduced and the commercial enzyme, respectively at the hydrolysis time of 90 min, enzyme dilution of 1.0,solid-liquid ratio of 30 g.L-1 and Tween 80 concentration of 1%.