Efficacy of connective tissue as a membrane with anorganic bone using platelet-rich plasma in the treatment of

Introduction: Regenerative periodontal therapy with a combination of platelet-rich plasma (PRP), anorganic bovine bone mineral (ABBM) and guided tissue regeneration (GTR) has been shown to result in significantly higher clinical attachment level gains compared to open flap debridement. However, there is no data on the efficacy of PRP in combination with connective tissue graft as a membrane. The aim of this study was to evaluate the efficacy of ABBM and connective tissue graft (CTG) as a membrane with and without PRP in the treatment of alveolar intrabony defects.Materials and Methods: In this double-blind randomized clinical trial 15 pairs of intrabony defects in 15 patients with chronic periodontitis were randomly treated with a combination of PRP+ABBM+CTG (test group) or ABBM+CTG (control group). The following clinical parameters were evaluated at baseline and at 6-month re-entry visit using a stent as reference point: probing pocket depth (PPD), clinical attachment level (PAL-S), free gingival margin position (FGM-S), crestal position (CREST-S), crest to defect depth and defect depth to stent. Independent t-test was used to compare parameter means between the two groups and paired t-test was used to compare the parameter means in each group before and after treatment.Results: No differences were observed in any of the parameters between baseline and 6-month re-entry between the two groups. At 6-month interval the test group showed mean PPD reduction of 2.8 ± 0.8 (p value < 0.0001), CAL gain of 2.5 ± 0. (p value < 0.0001) and bone fill of 2.6 ± 0.4 mm (p value < 0.0001).Conclusion: Based on the results of this study both treatment modalities were effective in the treatment of intrabony defects and PRP had no additional effect on improving the clinical parameters. Furthermore, connective tissue graft was a good alternative membrane in the treatment of intrabony defects.Key words: Connective tissue, Guided tissue regeneration, Periodontium, Platelet-rich plasma.