COMPARISON OF EVAPORATIVE LIGHT SCATTERING DETECTOR WITH UV DETECTION SYSTEM IN THE MOLECULAR MARKER COMPOUNDS ANALYSIS OF ASPARAGUS RACEMOSUS

Asparagus racemosus is known as Shatavari in Sanskrit. Dried roots of A. racemosus were extracted with organic solvents in different ratios and were analyzed using high performance liquid chromatography (HPLC) with Evaporative Light Scattering Detector (ELSD). The markers of our interest in A. racemosus are Shatavari I and Shatavari IV. As both the markers do not contain strong chromophore, it is difficult to identify it in the UV detection system of High performance liquid chromatography. Our research reports suggested that High Performance Liquid Chromatography (HPLC) with Evaporative Light Scattering Detector (ELSD) is used as an alternative and effective tool for the identification of the compounds which do not contain chromophores. Present study concludes that ELS Detection system is more efficient than the UV/Vis detection system.