General education, an educational idea relative to professional education, has entered into curriculum research stage in educational practices of Chinese universities. The purpose in this article is to construct a curriculum system for general education on Biotechnology. Firstly, the course background and significance related to Biotechnology in the general education are discussed by the integration of the practice on construction of the curriculum and subject matter in Biotechnology and Human in Shanghai Jiao Tong University. Subsequently, the ideas and techniques of construction of subject matters are discussed on the aspect of the course characters, subject matter content knowledge, method and organization, etc. Through the teaching practice for years, an educational website named as “Bitechnology and Its Application” has been set up, and relevant teaching materials have been published. This research will contribute to the exploration on other curricula construction on general education.