Development of a serotype colloidal gold strip using monoclonal antibody for rapid detection type Asia1 foot-and-mouth disease

In specificity and sensitivity assay, there was no cross-reaction of the antigen with the other type of FMD and SVDV. The detection sensitivity was found to be as high as 10-5 dilution of Asia1/JSL/05 (1 × 107.2TCID50/50 μL). There was excellent agreement between the results obtained by CGS and reverse indirect hemagglutination assay (RIHA), and the agreement can reach to 98.75%.We developed colloidal gold strips that have good qualities and does not require specialized equipment or technicians. This method provided a feasible, convenient, rapid, and effective for detecting type Asia1 FMDV in the fields.All cloven-hoofed species are susceptible to foot-and-mouth disease (FMD), and this disease is characterize by fever, vesicular lesions and erosion in the mouth and on the tongue, muzzle, feet and teats and cause great economic losses in the affected countries and they involve an extensive threat for rapid and wide spread [1]. FMDV as pathogeny of FMD is a member of the family Picormaviriae and exists in seven immunological distinct serotypes (Asia1, A, O, C, SAT1, SAT2 and SAT3). Incidence of type O, A and C has been recorded in different parts of the world, however, incidence of types Asia1 and SAT1 to 3 is mainly restricted to Asia1 and Southern Africa, respectively [2].During the Aisa1 type FMD outbreak in the China in 2005, the requirement for a 24 h slaughter policy did not allow sufficient time for laboratory confirmation of suspect infection following clinical diagnosis. A rapid test or field-based assay would be a valuable tool to initial diagnosis of FMDV in a suspect animal. Many of sensitive methods such as RIHA and RT-PCR have been developed to analyze FMDV in nasal swabs, epithelial suspensions and probangs of clinical samples submitted from the field or animals infected experimentally with cell culture [3,4]. Thus, it is extremely desirable to develop a rapid and convenient detection method for FMDV.The convenience and speed of the test have been achie