Effect of vitamin C on blood picture and some biochemical parameters of quail stressed by H2O2

This study was carried out to investigate the Vitamin C protective effect against the H2O2 – induced oxidative stress effects on some hematological and biochemical parameters of female quails (Coturnix coturnix).120 sexed female quails were reared from 21-56 days. Randomly the birds divided into 4 groups (30 birds/group) (2 replicates) as follows: 1st group: T1 (control): reared on standard ration and tap water.2nd group: T2 (H2O2 group): reared on standard ration and tap water supplemented with 0.5% H2O2. 3rdgroup: T3 (Vitamin C group): reared on standard ration supplemented with 300mg/kg ration and tap water. 4thgroup: T4 (H2O2 + Vitamin C): reared on standard ration supplemented with 300mg/kg ration and tap water supplemented with 0.5% H2O2. Results revealed that Vit. C supplementation improve female quails blood picture, this effects were adversive to the H2O2 effects, Vit. C causes a significant increase in lymphocytes % and a significant decrease in hetrophils and hetrophils: lymphocyte ratio (stress index), also a significant decrease in glucose, cholesterol and triglycerides, as well as a significant increase in serum protein when compared with the effect of H2O2 – induced oxidative stress. In conclusion, oxidative stress causes a negative effects on blood picture and some serum biochemical parameters, and Vit. C supplementation reduces and reverses the H2O2 effects.