Techniques of Mosquitoes Collecting and Laboratory Rearing

The field collecting and laboratory rearing of mosquitoes is essential for mosquito research. The mosquito specimens with perfect completeness and species richness are the foundation for extensive study on their biology, ecology, morphological taxonomy, molecular biology and insecticide resistance; however, the techniques for field colleting and laboratory rearing are still to be further improved and perfected. With many years of practice and improvement, the present paper introduces the ideal and operable tools of field collecting and laboratory rearing. Most importantly, here it summarizes in detail the collecting environment and method of mosquito larvae, pupae and adults, the techniques of the individual (with single-tube) and laboratory rearing of larvae and pupae and the progeny rearing of adults, the preservation method of larval and pupal exuviae and adults, and the field transportation method of all stages of mosquitoes. These technologies and methods are of instructive significance for mosquito field investigation and the acquirement of body-completed specimens and biological-ecological information.