Novel Model for Renal Failure and Anaemia Induced by 5/6 Nephrectomy in Wistar Rat

The present study describes modification in method of subtotal surgical renal ablation in rat and the advantageous effects of change in method in terms of biochemical and haematological parameters when compared to other established methods of nephrotoxicity. Male rats underwent 5/6 surgical nephrectomy or sham operations in two steps. The animals were divided into 3 groups viz. Group-I (Sham Control; n=6), Group-II (the whole right kidney ablation on day 1 and 2/3 left kidney ablation was done on day 7; n=10) and Group-III (the whole right kidney ablation and 2/3 leftt kidney ablation was done on same day; n=10). The haematological parameters like Haematocrit (HCT), Hemoglobin (Hb) and reticulocytes (%) and biochemical parameters like serum creatinine and urea were measured before surgery and on day 14, 21 and 28 after surgery There were significant increase in serum creatinine and urea levels in day 14, 21 and 28 post surgery suggestive of nephrotoxicity whereassignificantly decreased values of heamatocrit, haemoglobin and significantlyincreased value of reticulocytes suggest anaemia due to renal damage. Changes observed in the present study is comparable to the surgeries performed on the same day. There were more significant results in both surgery performed on the same day (Group –III) when compared to whole right kidney ablation and 2/3 left kidney ablation (Group –II) and Sham control (Group-I). There was only 20% mortality observed in group-III compared to 40 % in groupII. This model will be useful for the researcher tostudy nephotoxicity and anaemia, within short time, with less mortality, less animal suffering as per animal ethical point consideration and better values of biochemical and haematological parameters in comparison to the established models of 5/6 renal ablation.