SIMULTANEOUS ESTIMATION OF CURCUMIN AND QUERCETIN IN AYURVEDIC PROPRIETARY MEDICINE BY U.V. SPECTROPHOTOMETRY

A simple and reproducible U.V. spectrophotometric method for the quantitative determination of Curcumin and Quercetin in Madhujeevan churna (MJC) was developed and validated. The hydroalcoholic extract of developed churna was obtained by continuous heat extraction method. The method was validated using parameters such as linearity, precision, limit of detection, limit of quantification and recovery as per ICH guidelines. A new simple, rapid, sensitive, precise and economic spectrophotometric method in ultraviolet region has been developed for the determination of Quercetin and Curcumin in herbal formulation. The concentration of Quercetin and Curcumin present in raw material of madhujeevan churna was found to be 1.082 ± 0.011w/w and 2.163 ± 0.550 w/w respectively. The samples were prepared in ethanol and methods; obey Beers-Lamberts law in concentration ranges employed for evaluation. The result of analysis has been validated statistically and recovery studies confirmed the accuracy of the proposed method. Hence, the proposed method can be used for the reliable quantification of active marker compound in crude drug and its herbal formulations.