Screening of Antibacterial Activities of Marine Gastropod Hemifusus Pugilinus

In the present investigation was carried out to screen the antibacterial activities of marine gastropod Hemifusus pugilinus. The whole body of the animal was extracted in three different solvents such as, ethanol, methanol and water. The antibacterial properties were studied using 10 human pathogenic microorganisms such as, Escherichia coli, Klebsiella oxytoca, K. pnuemoniae, Lactobacillus vulgaris, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella typhi, S. paratyphi, Staphylococcus aureus, and Vibrio paraheamolyticus. The ethanolic extracts of H. pugilinus showed maximum antibacterial activities against E. coli (8 mm) and minimum activities against Vibrio paraheamolyticus (2 mm), Methanolic extracts showed highest activity in E. coli (6 mm) and lowest activity against S. paratyphi (1 mm) and the extract of water showed antibacterial activities against E. coli K. oxytoca and S. paratyphi. The crude extracts were purified in silica gel column chromatography with 11 fractions. In that E:M, 16:4, 12:8, 10:10, which showed maximum zone of inhibition against E.coli (4 mm), K. oxytoca (4 mm), K. pneumoniae (3 mm), L. vulgaris (4 mm) and V. parahemolyticus (5 mm). 18:2, 8:12, 6:14, 4:12 and 2:18 with these fractions showed minimum activities against the all pathogenic microbial forms.