Determination of Testes Regeneration Period for African Catfish (Clarias anguillaris) after Milt (Semen) Collection Through Ablation

The testes regeneration period of male African catfish (Clarias anguillaris) after milt collection through ablation was studied at 30 days intervals for the period 120 days with the intention to determine the testes regeneration period. Milt was collected from twelve matured males of Clarias anguillaris and the testes were left to regenerate. Testes weight, milt volume, Gonadosomatic Index (GSI), percentage fertilization and hatching rates increased with increase in testes regeneration periods. The highest testes weight, milt volume, gonadosomatic index, percentage fertilization and hatching rates were obtained at 120 days (4 months) regeneration period. There was no significant differences (p>0.05) between 120 and 90 days testes regeneration periods. Milt from African catfish Clarias anguillaris can be obtained without killing the male. The economical time to re-use male Clarias anguillaris after testes regeneration is 90 days (3 months). Fish breeders, hatchery operators as well as fish geneticist in Nigeria can therefore use male African catfish up to three times in a year for breeding purposes and for further genetic studies.