Evaluation of Genetic Diversity in Three Species of Retama Genus: R. monosperma (L) Boiss, R. raetam (Forssk) Webb and R. sphaerocarpa (L) Boiss. (Fabaceae) Based on SDS-PAGE

Genetic interpretation of protein polymorphism in Retama genus has not been well established until now. The aim of this study was to investigate interspecific and intraspecific polymorphism in three Species of Retama Genus: R. monosperma (L) Boiss, R. raetam (Forssk) Webb and R. sphaerocarpa (L) Boiss, by sodium dodecyl sulphate polyacrilamide gel electrophoresis (SDS-PAGE). In this study twelve populations collected from different regions of Algeria have been studied for the analysis of seed storage protein profiles to examine their relationship. Based on electrophoresis bans spectra, Jaccard’s Similarity Index was calculed and cluster analysis was performed using unweighted pair group method with arithmetical means (UPGMA), to check the polymorphism among populations. The results from the present study indicate that the low polymorphism between R. monosperma and R. raetam. The cluster analysis indicated that the twelve populations constituted two clusters. Cluster A comprised from all population of R. monosperma and R. raetam, and Cluster B comprised of 2 populations from R. sphaerocarpa. The SDS banding proteins pattern showed that four bands are monomorphic in all populations of three species with molecular weights of 31.761, 63.767, 92.363 and 82.832 KDa.