Potential of Aqueous and Alcohol Extracts of Quercus infectoria, Linusm usitatissium and Cinnamomum zeylanicium as Antimicrobials and Curing of Antibiotic Resistance in E. coli

Eighty two clinical isolates were collected from Sulaymani hospitals. The isolates show resistance to more than five antibiotics. The ethanol extracts of Quercus infectoria, Linusm usitatissium and Cinnamomum zeylanicium were proved to be the most powerful against E. coli E45 and E62 isolates, than aqueous extracts. The extracts exhibited most of the antibiotics activity against these two isolates irrespective of their antibiotic resistance behaviour. A comparative evaluation of plasmid elimination from E. coli E62 clinical isolate by sub-MIC of plant extracts showed that these extracts could cure plasmids effectively at their respective sub-MIC concentration. Maximum plasmid cured was observed by sub-MIC of ethanol extract of C. zeylanicum at 1100 :g/mL cured two plasmids 15 and 45 kb, followed by sub-MIC of L. usitatissimus extract at 1100 :g/mL was cured 45 kb plasmid and sub-MIC of aqueous and ethanol of Q. infectoria were cured 15 kb plasmid from E62 isolate. Curing of plasmid DNA from E. coli isolate confirmed by determining the loss of resistance markers in cured cultures.