Investigating Two Native Algal Species to Determine Antibiotic Susceptibility Against some Pathogens

The aim of present study was to investigate antimicrobial activity of two algal species Pithophora oedogonium and Botrydiopsis arhiza against four bacterial species: Salmonella 1 (egg), Salmonella 4 (meat 2), Staphylococcus sp., 4978 and Staphylococcus sp., 5034 and four fungal species viz: Aspergillus niger 419, Aspergillus flavus 1110, Penicillium viridicatum 1101 and Fusarium solini 1127. The algal cell mass was extracted in 100% ethanol and further concentrations of 2, 4, 6 and 8 mg/mL were made. Antagonistic effects were tested by using disc-diffusion method for bacterial species and agar well technique for fungal species. B. arhiza did not exhibit antimicrobial activity against all the test organisms. Whereas the ethanol extract of P. oedogonium inhibited the growth of two bacterial and two fungal species viz: Salmonella 1 (egg), Staphylococcus sp., 4978, F. solini and P. viridicatum. The maximum activity was observed against F. solini (22.3 mm) at 2 mg/mL conc. applied. It was noted that the growth of Salmonella 1 (egg) was inhibited maximally at 4 mg/m Lshowing zone of inhibition of 7, 4.67 and 4 mm at 6 and 8 mg/mL conc., respectively. The inhibitory zones of 7 and 3 mm were recorded for Staphylococcus sp., 4978 at 4 and 8 mg/mL conc. used. Among fungal species P. viridicatum showed maximum Zone of Inhibition (ZOI) of 7.6 mm at 8 mg/mL conc. The ethanolic extract of P. oedogonium can be efficiently used in antibiotics production as it proved effective against Salmonella 1 (egg), Staphylococcus sp., 4978, P. viridicatum and F. solini.