Isolation, Characterization and Comparative Study on Podophyllotoxin and Related Glycosides of Podophyllum heaxandrum

HPLC, column and thin layer chromatography guided studies led to the isolation of seven different compounds in methanolic extracts of Podophyllum hexandrum. The isolated compounds were analyzed using LC-MS and High Performance Liquid Chromatography (HPLC) studies interfaced to mass spectroscopy. Isolated compounds were used successfully as chemical markers for the comparison of the twelve different accessions of Podophyllum. We have also shown that the variation of chemical composition in P. hexandrum agree well with their botanical phylogeny as revealed by genetic phylogeny. HPLC analysis also revealed development of valuable chemotypes containing higher concentration of isolated marker compounds.