In vitro Differentiation Mode of Pancreatic Cells into Multiple Neuronal Phenotypes

The in vitro differentiation features of HPNE cells into neural cells were investigated. In this study, HPNE cells were pre-cultured in defined medium, and then induced by ITS treatment (insulin-transferrinselenium). After a transient ductal stage, HPNE cells present neural cell morphology. A series of neural markers were detected in terminally transformed HPNE cells. During HPNE transdifferentiation, the components of Notch signaling were elaborately modulated. These findings indicate that HPNE cells have the differentiation potential into neural cells.