Efficacy of the Precure Antimicrobial1 at Various pH Values for Controlling Microorganisms on Broiler Paws

The objective of the following study was to evaluate the most efficacious pH for treatment of post-chill, cleaned broiler paws with the Precure (Safe Foods Corporation, N. Little Rock, AR) antimicrobial. Precure is listed as a solution of GRAS acids for use by FDA and is listed as a safe and suitable ingredient by USDA for use on poultry. This study was done at the request of a poultry processor who was being asked by the customer to adhere to strict microbial standards for poultry paws. Thus, a bag of randomly collected, chilled and cleaned paws was obtained from a local broiler processing facility and was transported on ice to MCA Services (Rogers, AR). Upon arrival at the laboratory, the bag of paws was held frozen (< 28oF) for 2 days and was then tempered (40 to 42oF) for two days prior to initiation of the study. For the experiment, there was a control group and three treatment groups. The control group and each of the three treatment groups consisted of three replicate samples (n=3). Each replicate sample consisted of three randomly selected paws. Thus, a treatment group consisted of a total of nine paws. The three treatment groups evaluated were Precure at pH=1.5, Precure at pH=1.7 and Precure at pH=1.85. For treatment of the product, the nine paws for each treatment group were placed on a wire rack and were allowed to touch and overlap as would be typical in a processing environment. Each treatment group of paws was sprayed with the appropriate Precure treatment at 20 mL per second for 5 seconds. Thus, each group of nine paws was sprayed with 100 mL of the appropriate Precure treatment solution. The sprayed paws were then allowed to drain for 10 seconds. After draining, paws were placed three to a bag in sterile rinse bags and were held at 40oF for < 4 hours until initiation of the microbiological analyses. Each sample was evaluated for Aerobic Plate Count, coliforms and Escherichia coli, as well as for presence or absence of Salmonella in accordance with USDA/FSIS standard laboratory procedures using 100 mL Butterfield’s Phosphate Diluent. Petrifilm 3 was utilized for enumeration of organisms and Salmonella incidence was determined using the BAX 4 System PCR assay. The lower detection level for all quantified groups of organisms was 1 colony forming unit per mL. The control group of paws had an Aerobic Plate Count of 4.3 logs, a coliform count of 1.6 logs and an E. coli count of 1.6 logs. Two of the three control groups of paws were positive for Salmonella. Log reductions in Aerobic Plate Count were 0.3, 0.2 and 0.6 for the Precure treat