DEVELOPMENT OF AN ANALYTICAL METHOD TO DETERMINE EUGENOL CONCENTRATIONS IN ALCOHOLIC EXTRACTS OF DIFFERENT SPECIES OF OCIMUMa€ S

Eugenol is used as a flavor in the food industry, has a variety of biological activity, and can serve as a biomarker. Because eugenol is present in the leaves of Ocimum, which are used as a herbal medicines, a sensitive and reliable quantitative Ultra-violet spectroscopy and high-performance liquid chromatographic method has been established for quantification of the compound in the leaves of the plant. A methanol extract of the powder of dried leaves of Ocimum, was spotted on the Merck aluminium plate precoated silica gel F254 with 0.2 mm thickness. Meoh:Chloroform ( 95:5) and MeOH: H2O: ACN (50:25:75) used as mobile phase to isolate the eugenol and to prepare the sample for UV and HPLC analysis respectively.The UV and HPLC method proposed for the quantitative monitoring of eugenol in Ocimum leaf powder is rapid, simple, and precise. Hence from that UV and HPLC analysis it was conclude that the sanctum linn contains higher amount of Eugenol.