The effects of strontium chloride on viability of mouse connective tissue fibroblast cells

Aim. Strontium salts are effective and selective anti-irritants for chemically induced sensory irritation associated with stinging, burning, or itching. The aim of the present study was to determine the cytotoxic and/or proliferative effects of strontium chloride on fibroblast cell culture. Method. A mouse connective tissue fibroblast cell line, L929 (ATCC cell line, NCTC clone 929) was cultured. Fibroblast cell lines were examined with 20%, 10%, 5%, 2.5%, 1.25%, 0.6%, and 0.3% (w/v) concentrations of Strontium chloride hexahydrate (SrCl2.6H2O). The proliferation assay analyzed the number of viable cells by the cleavage of tetrazolium salts added to the culture medium, using the XTT labeling reagent. The optical density of the samples was compared with that of the control to obtain the percentage viability, as follows: cell viability (%)=[(OD450 (sample)/OD450 negative control))×100]. Results. The cytotoxicity value of strontium chloride for all concentrations (w/v) was compared with that of the control, and cytotoxicity levels were not higher than those of the controls (p>0.05). The level of viable cell was higher at 1.25% (w/v) than 2.5% (w/v) (p<0.05). Conclusion. Strontium chloride hexahydrate (SrCl2.6H2O) had no negative effect on cell viability at all the concentrations.