ANTIOXIDANT AND DNA BINDING STUDY 3,3'-(5,5'-METHYLENE BIS (3-MERCAPTO -4H-1,2,4-TRIAZOLE-5,4-DIYL)BIS(AZAN-1-YL-1- YLIDENE) DIINDOLIN-2-ONES

3,3'-(5,5'-methylene bis(3-mercapto-4H-1,2,4-triazole-5,4-diyl) bis (azan-1-yl-1-ylidene)diindolin-2-ones (VIIIa-t) have been synthesized by the condensation of an appropriate isatins(IIIa-t) with the intermediate 5, 51-methylene diyl bis (4-amino-4H- 1,2,4-triazole- 3-thiol)(VII). All the title compounds (VIIIa-t) were screened for Antioxidant activity and DNA Binding study, for this evaluation Ascorbic acid is employed as standard, for DNA binding Study employed DNA is Herring sperm DNA. Almost all the compounds showed free radicalscavenging activity and DNA binding to some extent. The structures of newly synthesized compounds were established on the basis of elemental analysis, IR, 1H NMR and mass spectral data.