Effects of Magnetic Field on the Electrophysiological Parameters of Soleus Muscle in Streptozotocin-Induced Diabetic Rats

Purpose: To investigate the effects of magnetic field (MF) on the resting membrane potential and action potential parameters of the Soleus muscle preparations in streptozotocin (STZ) induced diabetic rats. Methods: Forty male wistar rats were used in this study. The rats were divided into four groups; Group I (control), Group II (control with magnetic field), group III (diabetic), group IV (diabetic with magnetic field). The diabetes was induced by a single intravenous injection of STZ (45 mg/kg) in citrate buffer to the jugular vein under the ketamine and xylazine anesthetic combination. Resting membrane potential (RMP), muscle action potential (MAP), depolarization and half-repolarization time, MAP integral, depolarization and repolarization rates were recorded by means of the conventional microelectrode technique. Results: When group III and IV compared to group I, a significant decrease was found in the RMP (p<0.05). When control group compared to the other groups, MAP values found to be significant. When the depolarization time was compared there was a significant increase difference between group I and group III and group IV. Furthermore, half-repolarization time, MAP integral, depolarization and repolarization rates showed significant decrease results as occurred in MAP. Conclusion: MF increased the excitability of the cell membrane in RMP, which revealed that cell membrane had been more depolarized. It was assumed that this increase in depolarization may have been due to the changes in the conductivity of K+ and/or Cl- ion channels. [Cukurova Med J 2013; 38(3.000): 426-433]