Lornoxicam Inhibits Human Polymorphonuclear Cell Migration Induced by fMLP, Interleukin-8 and Substance P

It has been established that nonsteroidal anti-inflammatory drugs (NSAIDs) have additional mechanisms of action, besides the inhibition of prostanoid production. We evaluated the ability of lornoxicam, a novel NSAID of the oxicam family, to interfere with the migration of human polymorphonuclear cells (PMN) < I>in vitro < /I>. PMN were obtained from healthy donors. Chemotaxis was assessed by a Boyden microchemotaxis chamber and was stimulated with the bacterial chemoattractant formyl-met-leu-phe (fMLP, 0.1 M), the chemokine Interleukin-8 (25 ng mL < SUP>-1 < /SUP>, 0.003 M) or the neuropeptide substance P (0.1 M). Lornoxicam was used at the concentrations of 0.01, 1.0 and 100 M. This NSAID inhibited fMLP and Substance P chemotaxis starting from the concentration of 1.0 M, whereas Interleukin-8-induced chemotaxis was significantly reduced also at 0.01 M. As these concentrations can be easily reached in plasma and in the synovial fluid after the administration of this drug < I>in vivo < /I>, present data suggest that the inhibition of neutrophil migration is involved in the anti-inflammatory action of lornoxicam.