AN ION CHROMATOGRAPHIC METHOD FOR ESTIMATION OF METHANE SULPHONIC ACID IN GEMIFLOXACIN MESYLATE

The development and validation of an Ion chromatographic method for estimation of methane sulphonic acid in gemifloxacin mesylate drug substance was performed using acetone and buffer (1:9)as mobile phase and metrosep anion dual 2 as stationary phase at 0.5ml/min as flow rate. The analytes were monitored using suppressed conductivity detectors and linearity was obtained at the concentration range of 35.02 to 65.48 μg/ml and the correlation coefficient was found to be 0.9989.The recovery was found to be in the range of 103.53 to 116.30%w/w.