In vitro Propagation of Oncidium taka

The experiment was conducted at Tissue Culture Laboratory of Development of Biotechnology and Environmental Conservation Centre (DEBTEC), Dhaka, Bangladesh. Axillary buds of Oncidium taka were used as an experimental material for micropropagation. The effect of growth regulators concentrations on morphogenetic development was studied. Axillary bud produced protocorm on MS medium supplemented with combination of BA (0.5-1.5 mg L 1) and NAA (0.1-0.5 mg L 1). Satisfactory numbers of protocorm were observed at media with 1.0 mg L 1 BA + 0.5 mg L 1 NAA. Subsequent propagation from protocorm was performed on MS medium supplemented with various concentrations of BA or Kn and NAA or IBA. For Shoot proliferation, BA or Kn (1.0-3.0 mg L 1) and their combinations along with NAA (0.5-1.0 mg L 1) were used. Combination of cytokinin (BA and Kn) and Auxin (NAA or IBA) also obtained higher number of shoots and shoot length than cytokinin alone. The height shoot formation (9.4 1.14) and shoot length (5.1 0.74 cm) was observed at 2.0 mg L 1 BA + 1.0 mg L 1 NAA. For rooting IBA or NAA (0.5-2.0 mg L 1) and their combinations (0.5-2.0 mg L 1) along with coconut water (5%) were used. The highest numbers of roots were observed at NAA (1.0 mg L 1)+CW (5%). The plantlets were transferred in the pot and hardened properly.