Expression of Epitope Vaccine CTB-UA against Helicobacter Pylori in Transgenic Tomato

Helicobacter pylori is a key reason for Stomach diseases, and how to eradicate Helicobacter pylori has been studied for several decades. Oral vaccines produced by transgenic plants would change the traditional means of production and inoculation of Helicobacter pylori vaccines and reduce the vaccination cost significantly. In this research for the first time we used the transgenic tomato system to express Helicobacter pylori epitope vaccine CTB-UA, a recombinant peptide which could protect BALB/c mice from Helicobacter pylori infection after oral immunization by increasing the specific antibodies. CTB-UA was transformed into tomato mediated by Agrobacterium tumifient EHA105, and transgenic shoots have been verified by PCR analysis, RT-PCR and Enzyme-linked immunosorbent assay (ELISA) analysis showed that CTB-UA gene could transcript and produce the target peptide in the roots, fruits and leaves from the three transgenic tomato lines. Transgenic tomato derived oral vaccine strategy could be potentially used as an alternative strategy to fight for the H. pylori infection, especially for the people in developing worlds.