CHEMICAL COMPOSITION AND ANTIMICROBIAL ACTIVITY OF HARAMAYA PROPOLIS (BEE GLUE), ETHIOPIA

The present study was aimed at investigating the chemical composition and antifungal activity of Ethanol Extracted Haramaya propolis (EEHP). The GC-MS analysis of EEHP showed the presence of sixteen compounds and twelve compounds were identified by means of their retention times, by comparison of their mass spectra with the NIST 2005 library data and literature. The major constituents of EEHP were Benzenamine, N,N-dibutyl- (21.94%), Paromomycin(9.74%),4-Aminobutyramide,N-methyl-N-[4-(1-pyrrolidinyl)-2-butynyl]- (9.26%) and DL-Tryptophan,5-methoxy(7.43%). The Crude EEHP showed antifungal activities against Fusarium sp., Aspergillus niger and Colletotrichum sp.. GC-MS results showed that the antifungal activities of Haramaya propolis could be because of the presence of 3-(α-Hydroxyethyl)-aniline, Benzenamine,N,N-dibutyl, DL-Tryptophan,5-methoxy, 6-Amino-5,8-dimethoxyquinazoline, Paromomycin and Imidazole,2-fluoro-5-[2-carboxyvinyl].