Allele Frequency of Three Autosomal STR Loci D16S539, D7S820 and D13S317 in a Bangladeshi Population Sample

Allele frequency of three Short Tandem Repeats (STR) loci, D16S539, D7S820 and D13S317 were analyzed by PCR amplification and polyacrylamide gel electrophoresis in 52 unrelated Bangladeshi individuals. A GenePrint SilverSTR Triplex kit (Promega Corporation, USA) was used in this study. All STR loci were in Hardy-Weinberg equilibrium. Statistical parameters for forensic importance, the Power of Discrimination (PD), observed and expected Heterozygosity values (H), Polymorphism Information Content (PIC), Probability of Match (PM), Power of Exclusion (PE) and typical Paternity Index (PI) were calculated for the loci. These parameters indicated the usefulness of the loci in paternity testing and personal identification in Bangladeshi population. The combined Probability of Match (PM) and using the three loci was 3.88 x 10-4 and hence the PD (1-PM) was 0.99961. The combined power of exclusion for paternity for the three loci was also calculated at greater than 0.9396.