SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF FROVATRIPTAN SUCCINATE MONOHYDRATE IN BULK AND PHARMACEUTICAL DOSAGE FORMS

UV, first derivative, second derivative and AUC-spectrophotometric methods for the determination of Frovatriptan Succinate Monohydrate (FSM) in pharmaceutical formulations have been developed. For thefirst method, UV-spectrophotometry, standard solutions were measured at 280.2 nm. The linearity ranges were found to be 1.0–80.0 μg ml 1 in 0.1N NaOH and the regression equation was A=2.3214×10 2C-1.1543×10 2 (r=0.9995). For the second method, first derivative spectrophotometry, the response (dA/dλ) of standard solutions was measured at 294.0 nm. Calibration curve was constructed by plotting dA/dλ values against concentrations, 2.5–80.0μgml 1 of FSM standards in 0.1N NaOH. Regression equation of linear calibration graph was calculated as D1= -8.71×10 4C+5.17×10 4(r=0.9995). For the third method, second derivative spectrophotometry, the response (d2A/dλ2) of standard solutions was measured at 283.2 nm.Calibration curve was constructed by plotting d2A/dλ2 values against concentrations, 10.0–80.0μg ml 1 of FSM standards in 0.1N NaOH. Regression equation of linear calibration graph was calculated as D2= -9.8×10 5C –6.1×10 5 (r=0.9995). The fourth method was based on calculation of Area under Curve (AUC)for analysis of FSM in the wavelength range of 275.0–285.0 nm. Calibration curve was constructed by plotting AUC values against concentrations, 1.0–80.0μg ml 1of FSM standards in 0.1N NaOH. Regression equation of linear calibration graph was calculated as AUC=0.2287C-0.1229 (r=0.9995). The methods werevalidated by following the analytical performance parameters suggested by the International Conference on Harmonization. All validation parameters were within the acceptable range. The developed methods were successfully applied to estimate the amount of FSM in pharmaceutical formulations.