Sub-acute Toxicity Study of 3,6-Dimethyl-4-Ethyl-O-Acetyl Benzene Isolated from Soil Streptomyces Species

The sub-acute toxicity study of 3,6-dimethyl-4-ethyl-0-acetyl benzene (BM-5), was carried out on Long Evan`s rats using daily administration (300 g/rat/day) of compound for 14 consecutive days. Non-significant differences between weight of compound receiving rats and control rats (48 0.816 vs 46 0.816) were found. The change in hematological parameters was found to be nonsignificant (total count of RBC, 5.1 0.081 vs 4.63 0.094; white blood cell, 7.33 0.124 vs 5.73 0.124; platelets, 361666 10274 vs 261666 8498, (cell/ml) 106; percentage of hemoglobin 65.67 1.60 vs 56 0.816; ESR, 25 0.816 vs 19.67 0.471 for experimental and control rats, respectively) and biochemical parameters (serum glutamate pyruvate transaminase, 8.5 0.408 vs 8.33 0.471 IU L-1; serum glutamate oxaloacetate transaminase, 9.83 0.235 vs 9.33 0.234 IU L-1; bilirubin, 0.36 0.104 vs 0.34 0.016 g dL-1; creatinine, 0.67 0.089 vs 0.61 0.009 mg dL-1; urea, 18.83 0.235 vs 18.5 0.408 mg dL-1; for experimental and control rats). Therefore, the changes in body weight, hematological and biochemical parameters were statistically non-significant. No detectable abnormalities were found in histopathology of heart, kidney, liver and lung in experimental group of rats as compared with that of the control group of rats.