Optimization of Various Parameters for the Production of Glucose Oxidase from Rice Polishing Using Aspergillus niger

The enzyme glucose oxidase was produced by fermentation technology, using Aspergillus niger as fermentation organism and rice polishing as substrate. Maximum production of enzyme (3.42 U mL 1) was recovered at substrate level of 2% after 36 h of submerged fermentation. The pH for the optimal production of enzyme was found to be 4. Addition of salts such as urea (0.3%), CaCO3 (0.04%) and KH2PO4 (0.6%) into the fermentation medium enhanced enzyme production while MgSO4.7H2O was found to inhabit microbial growth and glucose oxidase production by A. niger.