In vitro Shoot Multiplication of Chrysanthemum morifolium as Affected by Sucrose, Agar and pH

Effects of sucrose, agar and pH on in vitro shoot multiplication of Chrysanthemum were studied. Nodal explant from the ex vitro grown plant was used as the test material. For optimum shoot induction and multiplication in MS medium containing BAP+ sucrose 30 gm l-1, agar 6 gm l-1 and pH 5.5-6.0 proved more effective. The media having 30 gm l-1 sucrose showed the highest percentage of explant responded to shoot proliferation and that was 100%. This sucrose concentration also showed the optimum result for number of usable shoots per culture, number of node shoot 1 and average length of shoots and the values were 5.4?0.6, 5.1?0.8 and 5.6?0.4 cm. The highest proliferation response of the explant was observed on MS medium having 6 gm l-1 of agar and the frequency was 100%. Among different level of pH, the highest percentage of explant showing proliferation was observed on the media adjusted to pH 5.5 and 6.0. The results presented here proved to be suitable for the in vitro shoot multiplication of Chrysanthemum morifolium.