A PCR-RFLP Method for the Analysis of Egyptian Goat MHC Class II DRB Gene

This study aimed to analysis the genetic polymorphisms of MHC class II DRB gene in the Egyptian goat using PCR-RFLP method. The amplified fragment with size of 285-bp was digested by two restriction enzymes TaqI and PstI. Restriction digestion of PCR product by TaqI enzyme represented two digested fragments at 122- and 163-bp (T restriction pattern) or undigested fragment at 285-bp (t restriction pattern). The frequencies of TT, Tt and tt patterns of MHC class II DRB in the Egyptian goats were 29.5, 61.4 and 9.1%, respectively. After the PstI digestion of the 285-bp PCR products amplified by Egyptian goat DNA, the results showed that the frequency of pp pattern (270- and 15-bp restricted fragments) was 29.5% and the frequency of heterozygous Pp pattern (270-, 226-, 44- and 15-bp restricted fragments) was 70.5%, while the PP pattern (226-, 44- and 15-bp restricted fragments) was not displayed in tested goat animals. The results declared that the p pattern (64.8%) is dominant over the P pattern (35.2%) in Egyptian goat.