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Biotechnology  2003 

Influence of Genotypes on Plant Regeneration from Cotyledons of Corchorus capsularis L.

Keywords: Corchorus capsularis , cotyledons , shoot regeneration , genotypes

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Abstract:

The present study was conducted on influence of genotypes on plant regeneration from cotyledons of Corchorus capsularis L. Multiple shoots were produced at the cut end of the petioles of each cotyledon of C. capsularis varieties on MS agar solidified medium supplemented by IAA and BAP. The results indicated that percentage of shoot regeneration from the cotyledon explants were found dependent on the genotypes used. Variation was observed among the varieties in number of cotyledons produced shoots and in number of shoots produced by each cotyledon. The highest number of shoots produced by each cotyledon and the highest number of cotyledons responded for shoot regeneration was recorded from Tri-cap-2. However, jute varieties have shown differences in in vitro responses and the differences were statistically significant. The results showed that the regenerated plants from all varieties were rooted in vitro and successfully transferred in pots containing soil. The plants grew well into maturity and produced seeds.

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