Pharmacognos tic and Phytochemical Studies on Gymnema sylvestre R. Br. Hairy V ariant

Gymnema sylvestre (Gs) is a well proven anti diabetic plant and used in Indian system of medicine for long period. A new variantof Gs was identified having hairy leaf. This Gs hairy variant species was subjected for pharmacognostical studies and screenedfor phytochemicalanalysisand correlated with the previous work done normal non hairy species. The leaves were analyzed formacroscopic and microscopiccharacteristics by taking microtome sectioning and stained for viewing in slides. The driedpowdered leaf was subjected to fluorescent study using chemical reagents and viewed under visible and UV light to study thecoloring pattern of the powdered particles interacting with the reagents. The powder was subjected for physicochemical studiesusing Indian Ayurvedic Pharamacopoeia procedure. The different extractions were done by using 100%,50% ethanol and water.These extracts were subjected for preliminaryqualitativeanalysis, quantitative estimation of terpenoides, alkaloids and markercompound Glycemnic acid. Further, TLC studies using different mobile phase and GCMS studies were carried out on 50%ethanolic extract. The stomatal frequency, index, vein islet number and palisade ratio values of Gs variant were varies incomparing with Gs normal. Test for flavanoides and, fixed oil and fats were given negative inferencein three extracts. Almost,other tests for compounds gave positive result in three extracts. Quantitative estimation results higher concentration ofterpenoides rather than alkaloids and presence of lower concentration of glycemnic acid comparing with previous work in extractof normal species. Chromatographic studies gave the presence of 33different compounds in 50% ethanolic extract can be used increating monograph for this species. All the results of pharmacognostical, physico, phytochemicalanalysisgave some variationwhile correlating with the results of same above studies on normal Gsspecies.