Ceftazidime and Vancomycin Sensitive Beta Hemolytic Staphylococcus aureus

Gram positive cocci, Staphylococcus aureus is a persistent nosocomial bacteria causing wide spectrum of infections in human. Its resistance to various groups of antibiotics is a worldwide public health concern. In view of this, a prospective study was undertaken to detect the microbial spectrum, hemolytic assay and antibiotic susceptibility of nosocomial bacteria. Air samples from different locations were collected by exposure plate technique and surface samples were collected by wiping the floor, wall, bed railing, patient table and curtain with sterile moist swabs. Collected samples were subjected to standard isolation and characterization procedures.Staphylococcus aureus was isolated from the collected samples. For hemolytic assay, Staphylococcus aureus culture was streaked on freshly prepared blood agar and incubated overnight. Hemolytic assay revealed beta hemolytic Staphylococcus aureus. Antimicrobial susceptibility test was performed on Staphylococcus aureus according to CLSI (2007) against gentamicin (120μg), vancomycin (30μg), erythromycin (15μg) tetracycline (30μg), ampicillin (10μg), amoxicillin (10μg) doxycycline (30μg), bacitracin (10μg) and ceftazidime (30μg). Staphylococcus aureus was sensitive to vancomycin and ceftazidime. Continuous education will generate better understanding about the usage of vancomycin and ceftazidime as first line antibiotic therapy to Staphylococcus aureus infection.