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EFFECT OF SOME AMINO ACIDS ON THE GROWTH AND L-GLUTAMIC ACID FERMENTATION BY AN AUXOTROPHIC MUTANT Micrococcus glutamicus AB100.

Keywords: L-glutamic acid , mutant , Micrococcus glutamicus , amino acids , synthetic medium]

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Abstract:

An investigation was carried out on the effect of some amino acids on the growth and production of Lglutamic acid by a mutant Micrococcus glutamicus AB100 using selected suitable synthetic medium. It wasobserved that among different amino acids studied, l-alanine, -arginine, l-threonine, l-tryptophan, l-proline and l-hitidine had positive effect; l-leucine, l-valine, l-phenylalanine, l-serine, l-methionine, l-lysine, l-glycine, lcystine and l-asparagine had no effect, where as l-glutamic acid had negative effect on growth and l-glutamic acid production. maximum growth and l-glutamic acid was obtained with l-alanine, 0.3 mg/ml; l-arginine, 0.5 mg/ml; l-threonine, 0.3 mg/ml; l-tryptophan, 0.5 mg/ml; l-proline 0.75 mg/ml; and l-hitidine, 0.75 mg/ml.

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