6. Spectrophotometric determination of tranexamic acid and mefenamic acid in tablet dosage form using derivatization technique

A new effective UV spectrophotometric method has been developed for the simultaneous estimation of tranexamic acid and mefenamic acid in combined tablet dosage form based on derivatization technique for making tranexamic acid UV detectable employing reaction of ninhydrin with primary amino group of tranexamic acid in the presence of basic medium via oxidation, deamination followed by condensation to form ruhemann purple product, quantitative determination is performed by measuring the absorbance at two different wavelengths 571 nm and 335 nm. Beer’s law is obeyed in the concentration ranging from 8- 40 μg/mL for tranexamic acid and 4- 20 μg/mL for mefenamic acid. The proposed method is validated for linearity, recovery, precision, robustness and specificity by statistical evaluation as per ICH guidelines. Tranexamic acid and mefenamic acid calibration curves are linear with correlation coefficient of 0.9983 and 0.9991. Recovery is between 98.0% - 101.0% for both tranexamic acid and mefenamic acid. The developed method can be used for routine quality control analysis of titled drugs combination in tablet formulation.