Comparative Pharmacological Study of Fruits and Flowers Extract of Withania somnifera

The in vitro antibacterial activity of Methanolic extracts of Withania somnifera (Solanaceae), was evaluated against seven Gram-negative bacteria, two Gram-positive bacteria and three fungi, using disk diffusion method followed by determination of minimum inhibitory concentrations by broth dilution method, against sensitive bacteria and fungi. Most of the extracts, at higher concentrations showed varying degrees of inhibitory activity against bacteria and fungi. The highest bio-activity was exhibited by the calyx extract against Pseudomonas aeruginosa. Staphylococcus aureus, Bacillus subtilis and Agerobacterium tumefaciens were the most sensitive pathogens after P. aeruginosa witch show maximum antimicrobial effects. Gentamycin and Ketoconazole, the standard antibiotics used were effective against the bacteria and fungi respectively.